A New Egyptian Satellite Strain of Cucumber Mosaic Cucumovirus

Biolistic inoculation of gladiolus with cucumber mosaic cucumovirus.

A new method of inoculation of gladiolus with cucumber mosaic virus (CMV) was developed using the Bio-Rad Helios Gene Gun System. This method circumvents the traditional use of aphids to transmit CMV, a virus that is mechanically transmissible to many plant species but only with difficulty to gladiolus. Cartridges containing virus-coated gold microcarriers were prepared and the virus shot into ...

A new strain of cucumber green mottle mosaic virus from iran

Efficient infection from cDNA clones of cucumber mosaic cucumovirus RNAs in a new plasmid vector.

Full-length cucumber mosaic cucumovirus (CMV) cDNAs were cloned into a new plasmid vector containing a modified plant virus promoter designed to transcribe the inserted sequence from its first nucleotide. cDNA copies of CMV strain Q (Q-CMV) genomic RNAs 1, 2 and 3 cloned into this vector were infectious when inoculated together, producing symptoms indistinguishable from those caused by wildtype...

Satellite RNA Associated with Cucumber Mosaic Virus

Helper Virus Independent Transcription and Multimerization of A 1 Satellite RNA Associated with Cucumber Mosaic Virus 2 3 Soon Ho Choi#, Jang-Kyun Seo#, Sun-Jung Kwon# and A. L. N. Rao* 4 Department of Plant Pathology & Microbiology, University of California, Riverside, 5 California 92521-0122 6 . 7 8 9 # These authors contributed equally to this work 10 11 12 Running title: Helper virus indepe...

Characterization of a satellite RNA associated with strain K8 of cucumber mosaic virus.

Cucumber mosaic virus strain K8 (K8-CMV), originally isolated in NW Italy from Cucumis metuliferus, was sent to us by Dr. Lisa after being passaged and multiplied in tobacco. Northern blots to probes against Fny-CMV (subgroup I) and Ls-CMV (subgroup II) showed K8-CMV to belong to subgroup I of CMV isolates (1). In agarose gel electrophoresis of virion encapsidated RNAs a fifth RNA was found, sh...